مشخصات مقاله

مشخصات مقاله
Rapid, Sensitive, and Validated HPLC Method for Analysis of Metronidazole and Tinidazole under Identical Chromatographic Conditions with UV Detection عنوان مقاله:
Original Article نوع مقاله:
نمایه شده در بانک‌های اطلاعاتی معتبر (درجه 1) اعتبار مقاله:
PHARMACOLOGY & PHARMACY موضوع مقاله:

Acta Chromatographica نام کامل مجله:
PMID:
0.79 Impact Factor:
2013-03-27 تاریخ انتشار:
ISSN چاپی:
ISSN الکترونیک:
شماره جلد:
شماره نسخه:
شماره صفحه شروع:
Label لینک مقاله:
شماره طرح پژوهشی:
5 نوع طرح پژوهشی:

خلاصه مقاله
Summary. A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid–liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze–thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.
cheat on wife developersalley.com why married men cheat on their wives
cheat on your wife site women cheat
reason why husband cheat how to know if wife has cheated why wife cheated
walgreens coupons in store myfreeadvice.co.uk rite aid coupon matchups
phone spy for android read spy cell phone app free
pharmacy direct coupon free manufacturer coupons coupons viagra
discount drug coupons discount prescription coupons pfizer coupons for viagra
coupons for viagra blog.brunothalmann.com canada drug pharmacy coupon
drug prescription card modelosguayaquil.com printable viagra coupon
viagra for sale uk viagra for sale uk viagra side effects blood pressure