مشخصات مقاله

مشخصات مقاله
Rapid, Sensitive, and Validated HPLC Method for Analysis of Metronidazole and Tinidazole under Identical Chromatographic Conditions with UV Detection عنوان مقاله:
Original Article نوع مقاله:
نمایه شده در بانک‌های اطلاعاتی معتبر (درجه 1) اعتبار مقاله:

Acta Chromatographica نام کامل مجله:
0.79 Impact Factor:
2013-03-27 تاریخ انتشار:
ISSN چاپی:
ISSN الکترونیک:
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شماره طرح پژوهشی:
5 نوع طرح پژوهشی:

خلاصه مقاله
Summary. A simple, rapid, and sensitive reversed-phase HPLC method was developed and validated for determination of metronidazole and tinidazole in human plasma samples under identical chromatographic conditions. This method involves liquid–liquid extraction using chloroform: isopropylalcohol (95:5). Chromatographic separation was performed using a μ-bondapack C18 (250 mm × 4.6 mm) column. The mobile phase consisted of potassium dihydrogen phosphate solution (0.005 M)/acetonitrile (80/20 v/v). The final pH of the mobile phase was adjusted to 4 ± 0.1 with orthophosphoric acid. The calibration curves were linear over the concentration range 0.1–15 μg/mL for metronidazole and tinidazole with the detection limit of 30 ng/mL. Within- and between-day precision and accuracy did not exceed 9.83% and 10.48%, respectively. Metronidazole and tinidazole were found to be stable in plasma samples with no evidence of degradation during 3 freeze–thaw cycles and 3 months storage in −70 °C. The current validated bio-analytical method was finally applied in bioequivalence studies of two different metronidazole and tinidazole products according to a standard two-way cross-over design with a two-week washout period. No statistically significant difference was observed between the logarithmically transformed AUC0-∞ and Cmax values. Therefore, generic products were considered bioequivalent with those of standards which could be used interchangeably.
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